Peptide Library Synthesis

Our Peptide Library Synthesis service is designed to produce hundreds of peptides in small quantities, tailored for high-throughput research and screening. This service is ideal for many applications such as epitope mapping, immuno-oncology, vaccine development, immunomonitoring… And also in quantitative proteomics, structure-activity relationship studies…

Peptide Library Synthesis – Quality Control Grades

At IC-PEPTIDE, we offer multiple levels of quality control to ensure the reliability and performance of your peptide libraries. Our available quality control methods include MS (Mass Spectrometry) and LC-MS (Liquid Chromatography coupled to Mass Spectrometry). In addition to confirm the presence of the expected peptide sequence, LC-MS also determines the purity level of each synthesized peptide. As quality control requirements can differ depending on your application, IC-PEPTIDE offers three quality control levels.

Peptide Library Synthesis – Modifications

IC-PEPTIDE libraries also offers a wide range of modifications such as the incorporation of non-natural amino acids, to restrict conformation by CLIPS technologies or disulfide bridges. Moreover, IC-PEPTIDE offers to add groups such as Biotin or fluorescent dyes (FAM, TAMRA, ..) and many others. We also offer heavy isotope labeling, so heavy peptides can be used for biomarker identification and quantification for instance. In conclusion several modifications are available depending on your specific demands: Phosphorylation (phosphorylated Serine, Threonine and Tyrosine), Biotinylation, Isotope labeling for heavy peptides, Addition of non-natural amino acids, others described below.

IC-PEPTIDE's exclusive benefits

In-silico pre-selection

The general rule in peptide synthesis is that the longest a sequence is, the lower the purity rate will be. Synthesis of a standard 10 amino acids peptide gave generally around 70% of purity rate. A 40 amino acid peptide will generally give a 30% purity rate. This is highly variable depending on amino acid composition and their positions. Knowing this, to maximize quality of syntheses, IC-PEPTIDE has developed a proprietary predictive tool to screen and select the easiest sequences to be synthesized. Thus, IC-PEPTIDE can select from a client's sequence-list peptides that should give best purity rate in synthesis.

Advantageous conditions for hit peptides

In case some peptides from a peptide library give good results and larger amount are needed, IC-PEPTIDE ensure a special discount on its individual peptide synthesis service to obtain milligrams to grams quantities.

Plate replication

IC-PEPTIDE can also synthesize your master plate and distribute the peptides in different plates so that you can store your product and use it when you need it.

Sample mixing

In order to build up your peptide libraries as well as possible, we can synthesize your plate(s) and gather the peptides in the same well. This action can be done manually or with the help of robot pipettors.

Applications of peptide libraries

IC-PEPTIDE libraries contains a large number of peptides and are used in many areas, such as biological assays and epitope mapping. But also in T-cell immunotherapy, immune monitoring, vaccine development and proteomics.

Illustration of possible peptide library designs

For example, you might be interested in our SARS-CoV-2 peptide libraries. IC-PEPTIDE synthesizes peptides in parallel batches of 96, ensuring consistency and efficiency. Available in multiple delivery formats (96-well plates / Microtubes / Custom), peptides are provided in quantities ranging from 2 µmol to 7 µmol per peptide.

Overlapping peptide library

Overlapping peptide libraries are used to figure out which part of a given protein or peptide contains the essential amino acids that contribute to its functionality. The process is defined by two parameters: peptide length and offset number. It is ideal for T-cell epitope identification.

Truncated peptide library

An amino acid is systematically removed from the N-term or C-term sequence. Truncated peptide libraries make it possible to identify the smallest sequence responsible for an activity of the peptide. Truncated peptide libraries are used to determine the role of the sequences surrounding key residues (determined by alanine scanning) and to identify peptides with enhanced proteolytic stability.

T-cell truncated peptide library

To further identify T-cell epitopes, T-cell truncated peptide libraries use overlapping designs in combination with truncated library designs. It allows to test all possible T-cell epitopes across a protein of interest in an efficient and rapid manner.

Heavy isotope peptide library synthesis

Peptides are used daily in quantitative proteomics. Thanks to isotopic labeling (12C can be replaced by 13C, 14N by 15N and 1H by 2H), the heavy peptide can be differentiated from other peptides of the same chemical formula by a higher mass. Traditional peptide libraries are used for screening. However, heavy peptides are used for absolute quantitation with selective reaction monitoring (SRM) or multiple reaction monitoring (MRM). In this way, IC-PEPTIDE's heavy isotope peptide libraries can be used as reference chemicals in targeted proteomics assays to measure the expression of selected proteins. Most common labeling are N-ter or C-ter K(13C 15N) or R(13C 15N). Other positions or amino acids can be considered. Purification options are also available for heavy isotope peptide libraries. To satisfy your requirements, we can also perform modifications on your heavy peptide library: CysCAM, Met[O], Phosphorylated-Serine-Threonine-Tyrosine, Biotinylation, acetylated Lysine, non natural amino acids, … For more information visit our amino acid chart and reference structure.